The aim of this study was to assess the antibacterial activity of poly(ε-caprolactone)/silver/silver ions (Ag) nanocomposite films prepared by using a roll-to-roll (r2r) direct writing technique . In this work, two different processes were used to produce the Ag nanocomposite films. The first was based on the r2r-direct writing technique that uses a sacrificial mold. Using this technique, the writing speed was estimated to be 15 mm/s. In the second process, a roll-to-roller (r2r) technique was used to produce films with higher speeds (up to 200 mm/s). The performance of the different films was tested using E. coli, Staphylococcus aureus and Pseudomonas aeruginosa. The results showed that the direct writing process produced high antibacterial activity on the nanocomposite films, whereas the r2r process did not. This result showed that lower Ag ion release could be obtained by using the r2r process because the lower the production speed, the lower the ability to produce nanocomposite films and therefore less Ag ions were released. This fact was also confirmed by using the first antibacterial test with E. coli, S. aureus and Pseudomonas aeruginosa, where the direct writing process produced the best antibacterial activity. On the other hand, the r2r technique produced stronger antibacterial activity for S. aureus and Pseudomonas aeruginosa but weaker antibacterial activity for E. coli. Finally, kinetic tests showed that the direct writing process produced more antibacterial activity than the r2r process for S. aureus and Pseudomonas aeruginosa but not for E. coli. These results showed that r2r process can be used for production of antibacterial films if they are required for a specific application .
The antibacterial activity of poly(lactic-co-glycolic acid) (PLGA) nanocomposite films loaded with AgNPs was evaluated against the Gram-positive bacteria Listeria monocytogenes (L. monocytogenes) and Staphylococcus aureus and the Gram-negative bacteria Salmonella enterica (Salmonella enterica) and Escherichia coli (E. coli).
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Chemical vapor deposition (CVD) is an effective and versatile fabrication method for the production of metallic nanomaterials and thin films, such as Au, Ag, Pt, and Ni. While the CVD method itself is well established, it has significant advantages for the deposition of thin films on various substrates, including non-planar surfaces. In this context, AuNPs have been deposited on substrates of various geometries. These include linear Si strips , single grooves , curved grooves , nano-pillars , and micro-pillars . The formation of linear AuNPs by the CVD method is discussed in this chapter. Linear AuNPs produced by CVD have found use in biological applications, such as the detection of microorganisms, and as well as sensors for measurement of environmental chemicals, such as polycyclic aromatic hydrocarbons (PAHs) . 827ec27edc